| Full name | adapter-ligated libraries of tRNA-derived sequences |
| Summary | Specifically designed for the detection of full-length tRNA molecules and tRNA-derived products in clinical tissue samples. Utiliing highly processive group II intron maturase MarathonRT. Adapter ligation strategy facilitated by molecular crowding, to capture both full-length species and RNA fragments already present in the assessed samples. Optimized strategy for mature tRNA quantification in samples with lower RNA qulity. |
| Key findings | Improvement of classification of tumor-associated tRNA signatures in clinical tissue samples. Identification of cell lineage- and tumor-specific tRNA profiles that effectively improve classification of oncogenic signatures in samples presenting higher levels of RNA fragmentation. |
| Sequencing strategy | RNA-Seq |
| Raw data | https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE186736 |
| Software/data repository | https://github.com/bioinfoUGR/sRNAtoolbox; https://github.com/sert23/ALLtRNAseq |
